化学
Online enrichment and separation of five flavonoids compounds from mikania micrantha using magnetic nanomaterials coupled with high speed countercurrent chromatography
2017-09-12
Mikania micrantha is one kind of invasive weeds.Flavonoids is generally existed in weeds.The approach to adsorbing and enriching flavonoids using magnetic nanoparticles has developed gradually in recent years,which takes advantage of magnetic properties to achieve rapid separation,sample operation.High-speed counter-current chromatography,being a support-free liquid-liquid partition method,eliminates irreversible adsorption of some elements of the sample onto the solid support,and has been widely used in the preparative separations of natural products.In this study,a new on-line method of magnetic nanoparticles(MNPs) coupled with high-speed countercurrent chromatography(HSCCC) using a same solvent system during the whole separation process was established to achieve the rapid separation of flavonoids from Mikania micrantha.The adsorption and desorption capacities of five different MNPs for flavonoid standards and Mikania micrantha crude extract were compared and the most suitable magnetic nanoparticle Fe_3O_4@SiO_2@DIH@EMIMLpro was selected as the on-line MNP column.An on-line separation system was established by combining this MNP column with HSCCC through a six-way valve.The solvent system ethyl acetate-methanol-water(25:1:25,v/v) was more suitable for simultaneously on-line separating three flavonoids quercetin-3-O-rutinoside,luteoloside and astragalin from Mikania micrantha.The purities of these three compounds with the ethyl acetate-methanol-water solvent system were 95.13%,98.54% and 98.19% respectively.
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中国第八届植物化感作用学术研讨会论文摘要集
2017年
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